A facile and sensitive immunoassay for the detection of alpha-fetoprotein using gold-coated magnetic nanoparticle clusters and dynamic light scattering.

نویسندگان

  • Changho Chun
  • Jinmyoung Joo
  • Donghoon Kwon
  • Chang Sup Kim
  • Hyung Joon Cha
  • Myung-Sub Chung
  • Sangmin Jeon
چکیده

A facile and sensitive immunoassay protocol for the detection of alpha-fetoprotein (AFP) was developed using gold-coated iron oxide magnetic nanoclusters and dynamic light scattering (DLS) methods. The increase in the average particle size due to AFP-mediated aggregation was measured using DLS, and the detection limit was better than 0.01 ng mL(-1).

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Fast protein detection using absorption properties of gold nanoparticles.

In this study we describe the use of gold nanoparticles as a fast detection system for the sensitive analysis of proteins. The immunological method allows for protein analysis at the nanogram level, as required for clinical diagnosis. Initially a test protein is used for the development of the assay. The system is subsequently adopted for alpha-fetoprotein, which is a relevant tumor marker. Thi...

متن کامل

Evaluation of Gold Nanoparticle Size Effect on Dose Enhancement Factor in Megavoltage Beam Radiotherapy Using MAGICA Polymer Gel Dosimeter

Background: Gold nanoparticles (GNPs) are among the most promising radiosensitive materials in radiotherapy. Studying the effective sensitizing factors such as nanoparticle size, concentration, surface features, radiation energy and cell type can help to optimize the effect and possible clinical application of GNPs in radiation therapy. In this study, the radiation sensitive polymer gel was use...

متن کامل

A facile method for the detection of DNA by using gold nanoparticle probes coupled with dynamic light scattering

In this paper, we present a simple and rapid method for deoxyribonucleic acid (DNA) detection using gold nanoparticle probes coupled with dynamic light scattering (DLS) analysis. The redox agent 1,4-dithio-dl-threitol cross-links the gold nanoparticles (AuNPs) to form clusters, while the monothiol DNA could terminate the formation and stabilize the assembled clusters by their negatively charge-...

متن کامل

Investigation of the Synthesis of Chitosan Coated Iron Oxide Nanoparticles under Different Experimental Conditions

Iron oxide (Fe3O4) nanoparticles with average sizes of 10 nm were synthesized by a chemical coprecipitation method in the presence of chitosan. Chitosan as a natural polymer which can be extracted from crustaceans was used in the synthesis process in order to achieve more dispersed nanoparticles. Also, chitosan was used to obtain functionalized magnetic nanoparticles for using in different area...

متن کامل

Vanadium Removal from Fuel Oil and ‎Waste Water in Power Plant Using Humic ‎Acid Coated Magnetic Nanoparticles

   A method for treating fuel oil and waste water of power plant is suggested which is including vanadium elimination through contacting with humic acid coated magnetic nano-adsorbent. The nano-adsorbent was modified with humic acid (HA) as a compound having carboxyl, hydroxyl and amin functional groups. HA/Fe3O4 nanoparticles were prepared by a co-precipitation procedure and were character...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Chemical communications

دوره 47 39  شماره 

صفحات  -

تاریخ انتشار 2011